An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons.

نویسندگان

  • Esther F A Brandon
  • Christiaan D Raap
  • Irma Meijerman
  • Jos H Beijnen
  • Jan H M Schellens
چکیده

The liver is the predominant organ in which biotransformation of foreign compounds takes place, although other organs may also be involved in drug biotransformation. Ideally, an in vitro model for drug biotransformation should accurately resemble biotransformation in vivo in the liver. Several in vitro human liver models have been developed in the past few decades, including supersomes, microsomes, cytosol, S9 fraction, cell lines, transgenic cell lines, primary hepatocytes, liver slices, and perfused liver. A general advantage of these models is a reduced complexity of the study system. On the other hand, there are several more or less serious specific drawbacks for each model, which prevents their widespread use and acceptance by the regulatory authorities as an alternative for in vivo screening. This review describes the practical aspects of selected in vitro human liver models with comparisons between the methods.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In vitro reduction of zearalenone to β-zearalenol by rainbow trout (Oncorhynchus mykiss) hepatic microsomal and post-mitochondrial subfractions

Mycoestrogen zearalenone (ZEA) is found in human foods and animal feeds. Its estrogenic potencymainly depends on its biotransformation fate. The hepatic biotransformation of ZEA in rainbow trout wasinvestigated in this study. Various concentrations of ZEA were separately incubated with the hepaticmicrosomal and post-mitochondrial sub-fractions in the presence of NADPH, and the metabolites wered...

متن کامل

Interspecies variation in the hepatic biotransformation of zearalenone: Evidence for bio-inactivation of mycoestrogen zearalenone in sturgeon fish

Zearalenone (ZEA) as mycoestrogen is found in human foods and animal feeds. Its estrogenic potency depends on its biotransformation fate. The hepatic biotransformation of ZEA in two species of sturgeon fish (Acipenser persicus and Huso huso) was investigated. ZEA was incubated with the hepatic microsomal and post-mitochondrial sub-fractions in the presence of NADPH and the metabolites were dete...

متن کامل

Interspecies variation in the hepatic biotransformation of zearalenone: Evidence for bio-inactivation of mycoestrogen zearalenone in sturgeon fish

Zearalenone (ZEA) as mycoestrogen is found in human foods and animal feeds. Its estrogenic potency depends on its biotransformation fate. The hepatic biotransformation of ZEA in two species of sturgeon fish (Acipenser persicus and Huso huso) was investigated. ZEA was incubated with the hepatic microsomal and post-mitochondrial sub-fractions in the presence of NADPH and the metabolites were dete...

متن کامل

Pros and Cons of Dancing in the Dark: Enhancing EFL learners' oral proficiency in an unplugged learning community

Recent thrust of research has focused on non-conventional classrooms for teaching to EFL learners. Amongst the newly developed models, Unplugged Classroom Model (UCM) is the most debated one as textbooks are aside and the syllabus is based on conversation-driven activities. This study sought to determine the pros and cons of unplugged teaching for enhancing EFL learners' oral proficiency. From ...

متن کامل

A Quick update from the Past to Current Status of Human Pluripotent Stem Cell-derived Hepatocyte culture systems

Pluripotent stem cells (PSCs) may be offered as an unlimited cell source for the hepatocyte generation. The generation of hepatocytes from stem cells in vitro would provide an alternative cell source for applications in drug discovery and cell transplantation. In this review, we discuss different approaches to generate pluripotent stem cell-derived hepatocytes, advantages, limitations for each ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Toxicology and applied pharmacology

دوره 189 3  شماره 

صفحات  -

تاریخ انتشار 2003